磷酸化肌球蛋白轻链6抗体
产品名称： 磷酸化肌球蛋白轻链6抗体
英文名称： phospho-MYL6(Tyr29+Ser30)
产品编号： hz-5478R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Rabbit Anti-phospho-MYL6(Tyr29+Ser30) antibody
| 产品编号 | hz-5478R |
| 英文名称 | phospho-MYL6(Tyr29+Ser30) |
| 中文名称 | 磷酸化肌球蛋白轻链6抗体 |
| 别 名 | ESMLC; LC 17; LC17; LC17 GI; LC17 NM; LC17A; LC17B; MLC 3; MLC-3; MLC1SM; MLC3; MLC3NM; MLC3SM; MYL 6; MYL6; MYL6_HUMAN; Myosin light chain 3; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin light chain A3; Myosin light chain alkali 3; Myosin light polypeptide 6 alkali smooth muscle and non muscle; Myosin light polypeptide 6; Smooth muscle and non muscle myosin alkali light chain; Smooth muscle and nonmuscle myosin light chain alkali 6; 17 kDa myosin light chain. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 心血管 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Pig, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MYL6 around the phosphorylation site of Tyr29+Ser30:IL(p-Y)(p-S)Q-NH2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. Function: Regulatory light chain of myosin. Does not bind calcium. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Subcellular Location: Cytoplasm. Nucleus. Similarity: Contains 3 EF-hand domains. SWISS: P60660 Gene ID: 4637 Database links: Entrez Gene: 4637 Human Entrez Gene: 17904 Mouse Entrez Gene: 396807 Pig Entrez Gene: 362816 Rat Entrez Gene: 685867 Rat Omim: 609931 Human SwissProt: P60661 Cow SwissProt: P60660 Human SwissProt: Q60605 Mouse SwissProt: P60662 Pig SwissProt: Q64119 Rat Unigene: 632717 Human Unigene: 337074 Mouse Unigene: 146093 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
 Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MYL6(Tyr29+Ser30) Polyclonal Antibody, Unconjugated(bs-5478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |