phospho-CTBP1 (Ser422),磷酸化C端结合蛋白1抗体

产品编号HZ-11275R
英文名称phospho-CTBP1 (Ser422)
中文名称磷酸化C端结合蛋白1抗体
别 名CTBP1 (phospho Ser422); p-CTBP1 (Ser422); CtBP1 (phospho S422); BARS; C terminal binding protein 1; CTBP; CTBP1; MGC104684; CTBP1_HUMAN; C-terminal-binding protein 1; CtBP1.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导 细胞周期蛋白 转录调节因子 细菌及病毒
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Danio rerio
phospho-CTBP1 (Ser422),磷酸化C端结合蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-CTBP1 (Ser422),磷酸化C端结合蛋白1抗体PubMedPubMed
产品介绍background:
CtBP1 associates in complexes with histone deacetylases (HDAC), the G9a histone methyltransferase, and LSD1 histone demethylase illustrating its role in transcriptional repression. Through its association with centrosomes, CtBP1 appears to also play a role in mitosis and Golgi complex organization.

Function:
Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.

Subunit:
Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217 (By similarity). Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:

Post-translational modifications:
The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
ADP-ribosylated; when cells are exposed to brefeldin A.
Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

Similarity:
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Database links:
UniProtKB/Swiss-Prot: Q13363.2

phospho-CTBP1 (Ser422),磷酸化C端结合蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.